METTL3/METTL14 Transactivation and m6A-Dependent TGF-β1 Translation in Activated Kupffer Cells
BACKGROUND AND AIMS: A) RNA modification participates in various cell stress responses, yet it remains unknown whether it plays a role in TGF-β1 upregulation in activated KCs. METHODS: A in TGF-β1 upregulation was further verified in METTL3/METTL14-deficient KCs and myeloid lineage cell-specific METTL14 knockout mice. RESULTS: A sites on the 5'UTR of TGF-β1 mRNA blocks LPS-induced increase of luciferase reporter activity. CONCLUSIONS: A modification in TGF-β1 upregulation, which helps to shed light on the molecular mechanism of NASH progression.