Targeted base editing in the plastid genome of Arabidopsis thaliana

著者: Issei Nakazato, Miki Okuno, Hiroshi Yamamoto, Yoshiko Tamura, Takehiko Itoh, Toshiharu Shikanai, Hideki Takanashi, Nobuhiro Tsutsumi, Shin‐ichi Arimura - Nature Plants 2021 被引用: 132

Abstract Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells 1 . We applied this system to targeted base editing in the Arabidopsis thaliana plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T 1 generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors.