Spatial organization and dynamics of RNase E and ribosomes in Caulobacter crescentus

著者: Camille Bayas, Jiarui Wang, Marissa K. Lee, Jared M. Schrader, Lucy Shapiro, W. E. Moerner - Proceedings of the National Academy of Sciences, PNAS 2018 被引用: 81

Significance Regulated gene expression involves accurate subcellular positioning and timing of RNA synthesis, degradation, processing, and translation. RNase E is an endoribonuclease that forms the scaffold of the RNA degradosome in bacteria, responsible for the majority of mRNA turnover and RNA processing. We used 3D superresolution microscopy and single-particle tracking to quantify the spatial distribution and dynamics of RNase E and ribosomes in the asymmetrically dividing bacterium Caulobacter crescentus . Our results show that active transcription and RNA substrate availability facilitate confinement and clustering of both RNase E and ribosomes. RNase E clusters colocalized with the subcellular position of the two Caulobacter rRNA gene chromosomal loci, indicating that RNA processing can be spatially organized in a bacterium according to its transcriptional profile.