Two‐Photon Excitation of Quantum‐Dot‐Based Fluorescence Resonance Energy Transfer and Its Applications

著者: Aaron R. Clapp, Thomas Pons, Igor L. Medintz, James B. Delehanty, Joseph S. Melinger, T. Tiefenbrunn, Philip E. Dawson, Brent Fisher, Brian O’Rourke, Hedi Mattoussi - Advanced Materials 2007 被引用: 120

Nonradiative fluorescence resonance energy transfer (FRET) between a luminescent quantum dot (QD) donor and a proximal dye brought in close proximity of the QD surface via conjugation with a dye-labeled peptide (or a protein) is shown (see figure). The system is excited with near IR irradiation (well below the absorption band of the QD), via a fast two-photon process, which produces a FRET signal with very low background contribution due to a substantially reduced nonlinear direct excitation of the dye.