Advances in the Application of Single-Cell Transcriptomics in Plant Systems and Synthetic Biology

著者: Md Torikul Islam, Yang Liu, Md Mahmudul Hassan, Paul E. Abraham, Jean Merlet, Alice Townsend, Daniel Jacobson, C. Robin Buell, Gerald A. Tuskan, Xiaohan Yang - BioDesign Research 2024 被引用: 36

spp.). It is expected that future technical advancements will reduce the cost of scRNA-seq and consequently accelerate the application of this emerging technology in plants. In this review, we summarize current technical advancements in plant scRNA-seq, including sample preparation, sequencing, and data analysis, to provide guidance on how to choose the appropriate scRNA-seq methods for different types of plant samples. We then highlight various applications of scRNA-seq in both plant systems biology and plant synthetic biology research. Finally, we discuss the challenges and opportunities for the application of scRNA-seq in plants.