Click Chemistry In Situ: Acetylcholinesterase as a Reaction Vessel for the Selective Assembly of a Femtomolar Inhibitor from an Array of Building Blocks

著者: Warren G. Lewis, Luke G. Green, Flavio Grynszpan, Zoran Radić, Paul R. Carlier, Palmer Taylor, M. G. Finn, K. Barry Sharpless - Angewandte Chemie 2002 被引用: 740

Form-fitting chemistry in a protein mold is enabled by the use of the 1,3-dipolar cycloaddition of azides and alkynes. The enzyme acetylcholinesterase preferentially assembles one pair of these reactants, each of which bears a group that binds to adjacent positions on the protein structure (see picture), into a 1,2,3-triazole adduct that is the most potent noncovalent inhibitor of the enzyme yet developed.