In Vivo Profiling and Visualization of Cellular Protein–Lipid Interactions Using Bifunctional Fatty Acids

著者: Per Haberkant, Reinout Raijmakers, Marjolein Wildwater, Timo Sachsenheimer, Britta Brügger, Kenji Maeda, Martin Houweling, Anne‐Claude Gavin, Carsten Schultz, Gerrit van Meer, Albert J. R. Heck, Joost C. M. Holthuis - Angewandte Chemie International Edition 2013 被引用: 154

Bifunctional lipid technology: Cells convert externally added photoactivatable and “clickable” fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid-bound proteins in situ (see scheme). As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.