Xenometabolite signatures in the UC Davis type 2 diabetes mellitus rat model revealed using a metabolomics platform enriched with microbe-derived metabolites

著者: Kelly E. Mercer, Laxmi Yeruva, Lindsay Pack, James L. Graham, Kimber L. Stanhope, Sree V. Chintapalli, Umesh D. Wankhade, Kartik Shankar, Peter J. Havel, Sean H. Adams, Brian D. Piccolo - American Journal of Physiology-Gastrointestinal and Liver Physiology 2020 被引用: 26

We debut a liquid chromatography-mass spectrometry (LC/MS) platform called the XenoScan, which is a metabolomics platform for xenometabolites (nonself-originating metabolites). This assay has 190 in-house standards with the majority enriched for microbe-derived metabolites. As a proof-of-principle, we used the XenoScan to discriminate genetic differences from cecal samples associated with different rat lineages, in addition to characterizing diabetes progression in rat model of type 2 diabetes. Complementing microbial sequencing data with xenometabolites uncovered novel microbial metabolism in targeted organisms.