Direct Measurement of Cerebrospinal Fluid Production in Mice

著者: Guojun Liu, Humberto Mestre, Amanda M. Sweeney, Qian Sun, Pia Weikop, Ting Du, Maiken Nedergaard - Cell Reports 2020 被引用: 131

ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.