A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

著者: Longzheng Chen, Wei Li, Lorenzo Katin‐Grazzini, Jing Ding, Xianbin Gu, Yanjun Li, Tingting Gu, Ren Wang, Xinchun Lin, Ziniu Deng, Richard McAvoy, Frederick G. Gmitter, Zhanao Deng, Yunde Zhao, Yi Li - Horticulture Research 2018 被引用: 180

plants, 17.2% were confirmed to be non-transgenic, for an overall non-transgenic mutation rate of 8.2%. Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction. This method should be applicable to many economically important, heterozygous, perennial crop species that are more difficult to regenerate.