Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies

著者: Taylor C. Stevenson, Colette Cywes‐Bentley, Tyler D. Moeller, Kevin B. Weyant, David Putnam, Yung‐Fu Chang, Bradley D. Jones, Gerald B. Pier, Matthew P. DeLisa - Proceedings of the National Academy of Sciences, PNAS 2018 被引用: 99

Significance A broad range of bacterial, fungal, and protozoan cells produce the surface polysaccharide poly- N -acetyl- d -glucosamine (PNAG), making this antigen an attractive target for vaccination against multiple human and economically important animal pathogens. While conjugate vaccines involving surface polysaccharides, such as PNAG, are a proven strategy for reducing the incidence of disease caused by bacterial pathogens, their manufacture is technically demanding, inefficient, and expensive, thereby limiting their widespread adoption. Here, we describe an alternative route to producing PNAG-containing glycoconjugates, whereby recombinant PNAG biosynthesis is coordinated with outer membrane vesicle formation in nonpathogenic Escherichia coli strains. The resulting glycosylated outer membrane vesicles effectively deliver PNAG antigens to the immune system while bypassing many of the drawbacks of conventional conjugate vaccines.