In Vitro Selection of Structure‐Switching Signaling Aptamers

著者: Razvan Nutiu, Yingfu Li - Angewandte Chemie 2005 被引用: 443

Picking out probes: A novel approach permits isolation of standard DNA aptamers by in vitro selection and conversion into fluorescence signaling probes. This method comprises the isolation of DNA aptamers capable of duplex-to-complex structure switching and labeling the derived aptamers with a pair of short DNA strands with a fluorophore F and a quencher Q to create a reporter system for real-time sensing (see picture).