Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow

著者: Alon Greenbaum, Ken Y. Chan, Tatyana Dobreva, David Alan Brown, Deepak Balani, Rogely Boyce, Henry M. Kronenberg, Helen J. McBride, Viviana Gradinaru - Science Translational Medicine 2017 被引用: 191

cells in their native three-dimensional environment. Our computational method counted all labeled osteoprogenitor cells without relying on sampling techniques and displayed increased precision when compared with traditional stereology techniques for estimating the total number of these rare cells. We demonstrate the value of the clearing-imaging pipeline by quantifying changes in the population of Sox9-tdTomato-labeled osteoprogenitor cells after sclerostin antibody treatment. Bone tissue clearing is able to provide fast and comprehensive visualization of biological processes in intact bone tissue.