Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Authors: John G. Doench, Nicolò Fusi, Meagan E. Sullender, Mudra Hegde, Emma W Vaimberg, Katherine Donovan, Ian C. P. Smith, Zuzana Tóthová, Craig B. Wilen, Robert C. Orchard, Herbert W. Virgin, Jennifer Listgarten, David E. Root - Nature Biotechnology 2016 cited by 4,983