Egr1 confers protection against acetaminophen‑induced hepatotoxicity via transcriptional upregulating of Acaa2

著者: Xiaohong Lei, Qingling Xu, Chunmin Li, Baolin Niu, Yanan Ming, Jing Li, Yingyue Tang, Xiaoyun Li, Jie Tang, Jing Wu, Yi Ju, Lvfeng Yao, Bin Wang, Qi Miao, Wei Zhong, Yang Zhi, Lirong Xu, Chao‐Jun Li, Xiaobo Li, Yimin Mao - International Journal of Biological Sciences 2022 被引用: 27

Background: Acetaminophen (APAP)-induced liver injury (AILI) is a common cause of drug-induced liver injury (DILI). The mechanism underlying protection in AILI or DILI remains to be elucidated, and the role of early growth response 1 (Egr1) in AILI and potential mechanisms remain to be known. Methods: The role of Egr1 was studied both in vivo and in vitro. Liver-specific Egr1-knockout (Egr1 LKO ) mice and those overexpressing Egr1 via tail vein injection of Egr1-expressing adenovirus (Ad-Egr1) were utilized with AILI. Chromatin immunoprecipitation-sequencing, RNA-sequencing, seahorse XF analysis, and targeted fatty acid analysis were performed. EGR1 levels were also studied in liver tissues and serum samples from AILI/DILI patients.